Scholarlink

The genomic analysis of circulating tumor cells (CTCs) can help us identify their specific characteristics and reveal intratumor heterogeneity while also reducing the need for invasive sampling. However, studying single CTCs can be challenging, as whole-genome amplification (WGA) is needed to obtain enough genetic material for high-throughput sequencing. Here, we present a protocol for isolating single CTCs from mouse xenografts, followed by WGA using the Ampli1WGA Plus kit.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Whole-Genome Amplification of Single Circulating Tumor Cells from Mice Xenografts

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Nuria Estévez-Gómez,
  • Cristóbal Fernández-Santiago,
  • Roberto Piñeiro,
  • David Posada

摘要

The genomic analysis of circulating tumor cells (CTCs) can help us identify their specific characteristics and reveal intratumor heterogeneity while also reducing the need for invasive sampling. However, studying single CTCs can be challenging, as whole-genome amplification (WGA) is needed to obtain enough genetic material for high-throughput sequencing. Here, we present a protocol for isolating single CTCs from mouse xenografts, followed by WGA using the Ampli1WGA Plus kit.