Scholarlink

Single-cell whole-genome sequencing (scWGS) has illuminated new perspectives on genetic diversity across different cellular conditions. Despite this, the amplification of DNA from sparsely populated formalin-fixed tissues during the whole-genome amplification (WGA) process remains a formidable challenge. We introduce an enhanced quality control protocol for WGA products generated using the multiple annealing and looping-based amplification cycles (MALBAC) technique. This approach presents a cost-efficient, high-throughput quality control method, delivering a precise assessment of WGA efficacy with 100% accuracy on a test set of 128 samples.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A Tailing Method of Quality Control on MALBAC-Based Whole-Genome Amplification Products

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Changyue Chen,
  • Jing Li,
  • ZengHui Xu

摘要

Single-cell whole-genome sequencing (scWGS) has illuminated new perspectives on genetic diversity across different cellular conditions. Despite this, the amplification of DNA from sparsely populated formalin-fixed tissues during the whole-genome amplification (WGA) process remains a formidable challenge. We introduce an enhanced quality control protocol for WGA products generated using the multiple annealing and looping-based amplification cycles (MALBAC) technique. This approach presents a cost-efficient, high-throughput quality control method, delivering a precise assessment of WGA efficacy with 100% accuracy on a test set of 128 samples.