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UV resonance Raman (UVRR) spectroscopy is a vibrational technique that provides molecular insights with high selectivity. By tuning the Raman excitation wavelength across the deep UV region, from 207 to 228 nm, the Raman signal of backbone or aromatic sidechains is selectively enhanced. This chapter describes the wavelength dependence of UVRR spectra and highlights the ability of 207 nm excitation to reveal the secondary structure of membrane-associated biomolecules.

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Deep UV Resonance Raman Spectroscopy: Applications to Membrane Proteins and Peptides

  • 实验方案

  • 发表日期：

    2026年

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  • Chanin B. Tangtartharakul,
  • Donovan G. Ruiz,
  • Judy E. Kim

摘要

UV resonance Raman (UVRR) spectroscopy is a vibrational technique that provides molecular insights with high selectivity. By tuning the Raman excitation wavelength across the deep UV region, from 207 to 228 nm, the Raman signal of backbone or aromatic sidechains is selectively enhanced. This chapter describes the wavelength dependence of UVRR spectra and highlights the ability of 207 nm excitation to reveal the secondary structure of membrane-associated biomolecules.