Lipid–protein interactions provide valuable insights into the functions of both lipids and proteins. A novel and powerful technique for identifying these interactions involves the use of protein microarrays combined with liposomes. Liposomes are spherical vesicles encapsulated by phospholipid bilayers, into which specific lipids of interest can be incorporated. This allows liposomes to serve as effective tools for detecting lipid–protein interactions and enables the simultaneous analysis of thousands of proteins with a limited number of lipids in a single experiment. This chapter outlines the methodologies and protocols for employing protein microarray assays alongside liposome fabrication to investigate protein–lipid interactions. Additionally, recent research findings in this field are reviewed.