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Antibodies with affinities in the lower nanomolar or sub-nanomolar range or specific off-rate binding kinetic (Koff) are favored for many research, diagnostic, and therapy applications. Many protocols for affinity maturation exist but often fail to produce desired results due to inefficient exploration of the sequence space or because the developability of the parental molecule is compromised. Here we describe a two-phase protocol to explore the sequence space for a given antibody efficiently, enabling simultaneous affinity and developability maturation.

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Simultaneous Affinity Maturation and Liability Removal from Antibodies

  • 实验方案

  • 发表日期：

    2026年

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  • Camila Leal,
  • Fortunato Ferrara,
  • Andrew Raymon Morton Bradbury,
  • Andre A. R. Teixeira

摘要

Antibodies with affinities in the lower nanomolar or sub-nanomolar range or specific off-rate binding kinetic (Koff) are favored for many research, diagnostic, and therapy applications. Many protocols for affinity maturation exist but often fail to produce desired results due to inefficient exploration of the sequence space or because the developability of the parental molecule is compromised. Here we describe a two-phase protocol to explore the sequence space for a given antibody efficiently, enabling simultaneous affinity and developability maturation.