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In this chapter, advanced methods for evaluating T-cell-dependent bispecific antibodies (TDBs) using in vivo fluorescence imaging and animal models are presented. TDBs, which target tumor-associated antigens on cancer cells and the TCR/CD3 complex on T cells, have shown success in hematologic malignancies but face challenges in solid tumors due to factors like reduced T-cell quality and low tumor-infiltrating T cells. Comprehensive protocols for cancer and T-cell preparation, flow cytometry, xenograft model generation, and imaging techniques are outlined, aiming to enhance the understanding of TDB dynamics and improve their efficacy in solid tumor treatment.

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Advanced Evaluation of T-Cell-Dependent Bispecific Antibodies Using Fluorescence Imaging and In Vivo Models

  • 实验方案

  • 发表日期：

    2026年

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  • Mikiko Itsukaichi,
  • Masahiro Yasunaga

摘要

In this chapter, advanced methods for evaluating T-cell-dependent bispecific antibodies (TDBs) using in vivo fluorescence imaging and animal models are presented. TDBs, which target tumor-associated antigens on cancer cells and the TCR/CD3 complex on T cells, have shown success in hematologic malignancies but face challenges in solid tumors due to factors like reduced T-cell quality and low tumor-infiltrating T cells. Comprehensive protocols for cancer and T-cell preparation, flow cytometry, xenograft model generation, and imaging techniques are outlined, aiming to enhance the understanding of TDB dynamics and improve their efficacy in solid tumor treatment.