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Iterative bleaching extends multiplexity (IBEX) is an easy-to-use, highly multiplex immunofluorescent tissue imaging method that employs widely available microscopy platforms, commercial reagents, and open-source software. In this article, we describe how to implement this method in a laboratory that has minimal experience with immunohistochemistry.

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High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX)

  • 实验方案

  • 发表日期：

    2026年

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  • Ian T. Lamborn,
  • Hiroshi Ichise,
  • Emily Speranza,
  • Leanne Arakkal,
  • Vivien I. Maltez,
  • Ziv Yaniv,
  • Andrea J. Radtke,
  • Ronald N. Germain

摘要

Iterative bleaching extends multiplexity (IBEX) is an easy-to-use, highly multiplex immunofluorescent tissue imaging method that employs widely available microscopy platforms, commercial reagents, and open-source software. In this article, we describe how to implement this method in a laboratory that has minimal experience with immunohistochemistry.