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Efferocytosis is a process in which specialized phagocytes, mainly macrophages, are responsible for recognizing and removing apoptotic cells from the inflammatory site, leading to tissue resolution, and preventing chronic inflammation. Herein we describe in detail a robust efferocytosis assay based on morphological criteria and flow cytometric for the quantification of efferocytic events and characterization of the engulfing efficiency of apoptotic cells by macrophage in vitro and in vivo, which can be used in many applications, including the development of genetic and pharmacological therapeutic targeting inflammation resolution.

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Quantitative In Vitro and In Vivo Assays to Assess the Efferocytic Efficiency of Macrophages

  • 实验方案

  • 发表日期：

    2026年

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  • Eduardo Gutseit,
  • Vinícius Amorim Beltrami,
  • Débora Gonzaga Martins,
  • Mauro Martins Teixeira,
  • Lirlândia Pires de Sousa,
  • Juliana Priscila Vago,
  • Vanessa Pinho,
  • Franciel Batista Felix

摘要

Efferocytosis is a process in which specialized phagocytes, mainly macrophages, are responsible for recognizing and removing apoptotic cells from the inflammatory site, leading to tissue resolution, and preventing chronic inflammation. Herein we describe in detail a robust efferocytosis assay based on morphological criteria and flow cytometric for the quantification of efferocytic events and characterization of the engulfing efficiency of apoptotic cells by macrophage in vitro and in vivo, which can be used in many applications, including the development of genetic and pharmacological therapeutic targeting inflammation resolution.