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The identification and exploration of microproteins encoded by long noncoding RNA (lncRNA) genes have the potential to revolutionize our understanding of human biology and expedite medical breakthroughs. Proteomics enables the identification and quantification of thousands of proteins present in cells, tissues or organisms, with LC-MS/MS serving as a pivotal technique in current proteomic research. Here, we describe a comprehensive suite of integrated methods to enhance proteomic analysis for the identification of lncRNA-encoded microproteins. This approach combines tricine gel electrophoresis, in-gel digestion, the establishment of a customized microprotein reference database, and LC–MS/MS analysis.

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Proteomic Identification of Microproteins Translated from Long Non-coding RNA

  • 实验方案

  • 发表日期：

    2026年

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  • Jing Zhang,
  • Zi-Hong Fan,
  • Qing-Yu He,
  • Yang Wang

摘要

The identification and exploration of microproteins encoded by long noncoding RNA (lncRNA) genes have the potential to revolutionize our understanding of human biology and expedite medical breakthroughs. Proteomics enables the identification and quantification of thousands of proteins present in cells, tissues or organisms, with LC-MS/MS serving as a pivotal technique in current proteomic research. Here, we describe a comprehensive suite of integrated methods to enhance proteomic analysis for the identification of lncRNA-encoded microproteins. This approach combines tricine gel electrophoresis, in-gel digestion, the establishment of a customized microprotein reference database, and LC–MS/MS analysis.