Medicinal mushrooms are renowned for their bioactive compounds, polysaccharides, triterpenoids, phenolic compounds, and ergosterol that support the body’s detoxification processes. Given the increasing environmental toxin exposure, medicinal mushrooms offer a promising solution for natural detoxification, supporting the liver, kidneys, and immune system. Their potential for heavy metal chelation and oxidative stress reduction makes them valuable components of modern detox protocols. By elucidating the underlying biochemical pathways, this research can contribute to a deeper understanding of the mechanisms by which medicinal mushrooms exert their detoxifying effects. The proposed protocol involves in vitro and in vivo studies, including cell-based assays, animal models, and biochemical analyses. The findings from this research could lead to the development of novel therapeutic strategies for detoxification and related disorders.