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The unintended exposure to compounds interfering with the estrogen hormone system can pose a threat to human health and the environment. In this chapter, we describe the E-Morph Screening Assay, a targeted imaging-based high-throughput phenotypic screening assay that allows the efficient identification and characterization of such compounds with estrogenic or anti-estrogenic activities in a 96- or 384-well plate format. It captures the phenotypic response of MCF-7 breast cancer cells, which comprise a complete, functional, and interconnected estrogen signaling pathway. The E-Morph Screening Assay measures the change of E-Cadherin-GFP signal intensity at adherens junctions as a quantitative readout for estrogen-dependent phenotypic changes in the morphology of cell–cell contacts.

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The E-Morph Screening Assay: Phenotypic Screening for Estrogenic or Anti-estrogenic Compound Activities

  • 实验方案

  • 出版模式：

    开放获取

  • 发表日期：

    2026年

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  • Ann-Sophie Aßmann,
  • Sebastian Dunst,
  • Verena Fetz

摘要

The unintended exposure to compounds interfering with the estrogen hormone system can pose a threat to human health and the environment. In this chapter, we describe the E-Morph Screening Assay, a targeted imaging-based high-throughput phenotypic screening assay that allows the efficient identification and characterization of such compounds with estrogenic or anti-estrogenic activities in a 96- or 384-well plate format. It captures the phenotypic response of MCF-7 breast cancer cells, which comprise a complete, functional, and interconnected estrogen signaling pathway. The E-Morph Screening Assay measures the change of E-Cadherin-GFP signal intensity at adherens junctions as a quantitative readout for estrogen-dependent phenotypic changes in the morphology of cell–cell contacts.