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Germination is a highly dynamic developmental transition from dry quiescent seeds to vegetative seedlings which involves major chromatin rearrangements and changes in transcriptional programs. Assay for transposase-accessible chromatin with sequencing (ATAC-seq) is a quick and reproducible method for quantifying relative chromatin accessibility genome-wide. In this chapter, we describe the use of ATAC-seq for profiling chromatin accessibility changes and differential transcription factor binding site accessibility during germination from enriched embryos.

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Identification of Accessible Transcription Factor Binding Sites During the Seed-to-Seedling Transition

  • 实验方案

  • 发表日期：

    2026年

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  • Benjamin J. M. Tremblay,
  • Julia I. Qüesta

摘要

Germination is a highly dynamic developmental transition from dry quiescent seeds to vegetative seedlings which involves major chromatin rearrangements and changes in transcriptional programs. Assay for transposase-accessible chromatin with sequencing (ATAC-seq) is a quick and reproducible method for quantifying relative chromatin accessibility genome-wide. In this chapter, we describe the use of ATAC-seq for profiling chromatin accessibility changes and differential transcription factor binding site accessibility during germination from enriched embryos.