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Proximity labeling (PL) techniques have emerged as powerful approaches for the study of protein associations in vivo. In this chapter, we present a step-by-step TurboID-based PL protocol to define the “proxiome” of a protein of interest in plants, from the generation of the fusion protein to the analysis of the results, using the publicly available Ti-TAN plasmid collection.

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TurboID-Based Proximity Labeling in Plants

  • 实验方案

  • 发表日期：

    2026年

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  • Chaonan Shi,
  • Huang Tan,
  • Rosa Lozano-Durán

摘要

Proximity labeling (PL) techniques have emerged as powerful approaches for the study of protein associations in vivo. In this chapter, we present a step-by-step TurboID-based PL protocol to define the “proxiome” of a protein of interest in plants, from the generation of the fusion protein to the analysis of the results, using the publicly available Ti-TAN plasmid collection.