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The Luminex multiplex bead immunoassay enables the detection of more than 100 analytes in a sample at a time. This method utilizes magnetic beads coated with antibodies of interest to measure multi-analytes simultaneously. Here, we describe a detailed protocol for multi-analyte detection of two proteins, fatty acid binding protein 1 (FABP1) and fibroblast growth factor 19 (FGF19), both related to fat metabolism, in cell lysates of HepG2 hepatocytes.

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Detection of Multi-analytes Using Luminex Multiplex Bead Immunoassay

  • 实验方案

  • 发表日期：

    2026年

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  • Md Asrarul Islam,
  • Yamin Farabih,
  • Sunil Kumar

摘要

The Luminex multiplex bead immunoassay enables the detection of more than 100 analytes in a sample at a time. This method utilizes magnetic beads coated with antibodies of interest to measure multi-analytes simultaneously. Here, we describe a detailed protocol for multi-analyte detection of two proteins, fatty acid binding protein 1 (FABP1) and fibroblast growth factor 19 (FGF19), both related to fat metabolism, in cell lysates of HepG2 hepatocytes.