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IFNγ is a type II interferon with both anti-tumor and tumor-promoting functions. Since IFNγ is produced in response to cancer chemotherapy and immunotherapy, it is critical to understand its tumor-promoting mechanisms. In ovarian cancer (OC), a short term IFNγ exposure inhibits OC cell invasion, while longer-term exposure induces OC cell invasion; the underlying mechanisms involve IFNγ-induced IL-8 expression. Here, we describe a 3D spheroid culture assay to analyze IFNγ-induced invasion in ovarian cancer cells. The main advantages of this assay include its relevance to tumor biology, reproducibility, and ease of cell manipulations and visualization.

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Analysis of IFNγ-Induced Invasion of Ovarian Cancer Cells Using 3D Spheroids

  • 实验方案

  • 发表日期：

    2026年

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  • Bijaya Gaire,
  • Ivana Vancurova

摘要

IFNγ is a type II interferon with both anti-tumor and tumor-promoting functions. Since IFNγ is produced in response to cancer chemotherapy and immunotherapy, it is critical to understand its tumor-promoting mechanisms. In ovarian cancer (OC), a short term IFNγ exposure inhibits OC cell invasion, while longer-term exposure induces OC cell invasion; the underlying mechanisms involve IFNγ-induced IL-8 expression. Here, we describe a 3D spheroid culture assay to analyze IFNγ-induced invasion in ovarian cancer cells. The main advantages of this assay include its relevance to tumor biology, reproducibility, and ease of cell manipulations and visualization.