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We describe here the application of ultrastructure expansion microscopy (U-ExM) to Trypanosoma cruzi. This super-resolution technique increases the spatial resolution of a cell or tissue for microscopic imaging by physically expanding a sample with off-the-shelf chemicals and common lab equipment. As the complete T. cruzi life cycle can be recapitulated in the lab using different cell culture techniques, we describe a detailed protocol for the application of U-ExM in all life cycle stages in vitro.

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Ultrastructure Expansion Microscopy for the Analysis of Trypanosoma cruzi Infection

  • 实验方案

  • 发表日期：

    2026年

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  • Felipe Castro Capurro,
  • Victoria Lucia Alonso

摘要

We describe here the application of ultrastructure expansion microscopy (U-ExM) to Trypanosoma cruzi. This super-resolution technique increases the spatial resolution of a cell or tissue for microscopic imaging by physically expanding a sample with off-the-shelf chemicals and common lab equipment. As the complete T. cruzi life cycle can be recapitulated in the lab using different cell culture techniques, we describe a detailed protocol for the application of U-ExM in all life cycle stages in vitro.