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Dual-wavelength ratiometric fluorescent probes are the current gold standard for generating a quantitative measure of subcellular compartment ion contents. This chapter describes the use of fluorescent probes to measure lysosomal pH, encompassing probe selection, calibration, instrumentation, and the miniaturization of assays to use for high-throughput readouts.

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Lysosomal pH Measurements via Dual-Imaging Microscopy

  • 实验方案

  • 发表日期：

    2026年

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  • Emily Kirkham,
  • Sian Gardiner,
  • Emyr Lloyd-Evans,
  • Hannah Best,
  • Helen Waller-Evans

摘要

Dual-wavelength ratiometric fluorescent probes are the current gold standard for generating a quantitative measure of subcellular compartment ion contents. This chapter describes the use of fluorescent probes to measure lysosomal pH, encompassing probe selection, calibration, instrumentation, and the miniaturization of assays to use for high-throughput readouts.