FRIL, a mannose/glucose binding lectin, from lablab purpureus or hyacinth beans exhibits therapeutic and biomedical applications including antibacterial, antifungal, and anti-SARS-Cov-2 activities, due to its higher affinity toward carbohydrates. In addition to its antimicrobial activity, FRIL protein was able to preserve primitive hematopoietic and neural progenitors from differentiation in suspension culture for a month without changing the medium. In this chapter, we illustrate the procedure to extract this valuable protein directly from the seeds of Lablab purpureus. The protein extraction techniques include homogenization, ammonium sulfate precipitation and anion exchange chromatography. The obtained pure protein was biochemically characterized using hemagglutination and hemagglutination-inhibition assays.