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Phytosterols are plant-derived sterols known for their cholesterol-lowering and therapeutic effects. This chapter focuses on the downstream processes for isolating, purifying, and analyzing phytosterols from crude extracts. Extraction protocols are described in Chapter 4 . The methods covered include recrystallization, urea complexation, molecular distillation, membrane filtration, and preparative HPLC for purification, along with TLC, GC-MS, HPLC, NMR, and FTIR for structural characterization. These methods facilitate the production of high-purity phytosterols for food, pharmaceutical, and analytical applications.

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Isolation and Characterization of Health Promoting Phytosterols

  • 章节

  • 发表日期：

    2026年

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  • Pragya Pandey,
  • Pintu Choudhary,
  • Simardeep Kaur,
  • Kuldeep Pandey,
  • Jagveer Singh,
  • Amarjeet Kaur

摘要

Phytosterols are plant-derived sterols known for their cholesterol-lowering and therapeutic effects. This chapter focuses on the downstream processes for isolating, purifying, and analyzing phytosterols from crude extracts. Extraction protocols are described in Chapter 4 . The methods covered include recrystallization, urea complexation, molecular distillation, membrane filtration, and preparative HPLC for purification, along with TLC, GC-MS, HPLC, NMR, and FTIR for structural characterization. These methods facilitate the production of high-purity phytosterols for food, pharmaceutical, and analytical applications.