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This procedure provides instructions for detection of Cryptosporidium from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium deoxyribonucleic acid (DNA).

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Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

  • 实验方案

  • 发表日期：

    2026年

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  • Amy M. Kahler,
  • Mia C. Mattioli

摘要

This procedure provides instructions for detection of Cryptosporidium from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium deoxyribonucleic acid (DNA).