Culture and Electrophysiological Analysis of Patient-Specific iPSCs Using Microelectrode Array Dishes
摘要
Microelectrode arrays offer an exciting opportunity to probe and characterize the functional status of dystrophin-deficient muscle tissue vis-à-vis patient-specific iPSC-derived skeletal muscle (SkM) and cardiac muscle (CM). Here, we present a method to culture and analyze baseline electrophysiological profiles of Duchenne muscular dystrophy (DMD) iPSC-SkM and iPSC-CM using MEA dishes.