Scholarlink

Southern hybridization is a method for detection of specific DNA sequences and gene analysis. It is performed by electrophoresis of the DNA samples after digesting with an appropriate restriction enzyme and transferring it to a nylon membrane and hybridizing it with a labeled probe. In this chapter we describe the protocol for transgene detection in rice plants by capillary transfer of DNA to a nylon membrane and hybridizing it with a Digoxin-labeled oligonucleotide probe and detection using alkaline phosphatase conjugated anti DIG antibody.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Characterization of Transgenic Rice Plants by Non-radioactive Southern Hybridization

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Rajasekaran Sripriya,
  • Mahitha Koonjangadan Padinjhareveedu,
  • Ajith Thaliyakkattil Girijan,
  • Jasmine M. Shah

摘要

Southern hybridization is a method for detection of specific DNA sequences and gene analysis. It is performed by electrophoresis of the DNA samples after digesting with an appropriate restriction enzyme and transferring it to a nylon membrane and hybridizing it with a labeled probe. In this chapter we describe the protocol for transgene detection in rice plants by capillary transfer of DNA to a nylon membrane and hybridizing it with a Digoxin-labeled oligonucleotide probe and detection using alkaline phosphatase conjugated anti DIG antibody.