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Recent studies have established a key role of inositol pyrophosphates (PP-InsPs) in regulating phosphate (Pi) homeostasis across various organisms. In plants, PP-InsP levels are intricately linked to Pi status, with this reciprocal regulation being especially pronounced under fluctuating Pi conditions compared to stable nutrient sufficiency or deficiency. Here, we present a hydroponic cultivation protocol for Arabidopsis and rice, designed to simulate distinct and fluctuating Pi regimes. The composition of the nutrient solutions in these systems can be easily adjusted, enabling precise monitoring of changes in PP-InsP metabolism, under both steady-state and dynamic Pi conditions. Plants grown hydroponically under different Pi regimes can also be analyzed for phosphate starvation response (PSR) phenotypes and subjected to inositol phosphate (InsP)/PP-InsP extraction and quantification using methods detailed in other chapters of this special issue.

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Hydroponic Cultivation Systems to Study Nutrient-Dependent Changes in Inositol Polyphosphate Metabolism in Plants

  • 实验方案

  • 发表日期：

    2025年

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  • Verena Gaugler,
  • Lukas Krusenbaum,
  • Esther Lange,
  • Shyam Pariyar,
  • Dhirendra Fartyal,
  • Mathias Becker,
  • Matthias Wissuwa,
  • Ricardo F. H. Giehl,
  • Gabriel Schaaf,
  • Marília Kamleitner

摘要

Recent studies have established a key role of inositol pyrophosphates (PP-InsPs) in regulating phosphate (Pi) homeostasis across various organisms. In plants, PP-InsP levels are intricately linked to Pi status, with this reciprocal regulation being especially pronounced under fluctuating Pi conditions compared to stable nutrient sufficiency or deficiency. Here, we present a hydroponic cultivation protocol for Arabidopsis and rice, designed to simulate distinct and fluctuating Pi regimes. The composition of the nutrient solutions in these systems can be easily adjusted, enabling precise monitoring of changes in PP-InsP metabolism, under both steady-state and dynamic Pi conditions. Plants grown hydroponically under different Pi regimes can also be analyzed for phosphate starvation response (PSR) phenotypes and subjected to inositol phosphate (InsP)/PP-InsP extraction and quantification using methods detailed in other chapters of this special issue.