Scholarlink

Enteric glia are important regulators of the gastrointestinal homeostasis in health and disease. To better understand these processes, it is necessary to be able to isolate and study enteric glia. Here we provide a protocol that expands upon conventional methods of glial cell isolation with the addition of microfluidic cell sorting, exploiting the glia-specific expression of the ACSA2 surface protein. Isolated live cells can be used for a number of downstream processes, such as cell culturing and/or proteomic or genomic analysis. Thus, the detailed isolation methods used are potentially effective ways of isolating and enriching enteric glia.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fluorescence-Activated Cell Sorting of Primary Enteric Glia

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Austin Irwin,
  • Anthony Pellicano,
  • Vladimir Grubišić

摘要

Enteric glia are important regulators of the gastrointestinal homeostasis in health and disease. To better understand these processes, it is necessary to be able to isolate and study enteric glia. Here we provide a protocol that expands upon conventional methods of glial cell isolation with the addition of microfluidic cell sorting, exploiting the glia-specific expression of the ACSA2 surface protein. Isolated live cells can be used for a number of downstream processes, such as cell culturing and/or proteomic or genomic analysis. Thus, the detailed isolation methods used are potentially effective ways of isolating and enriching enteric glia.