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This chapter describes easy and cheap methods for the long-term storage of wild-type and engineered Komagataella phaffii strains. When performed correctly, the outlined cryopreservation protocols allow almost indefinite storage of cells without losing their genetic integrity or viability.

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Cryopreservation of Pichia pastoris Strains for Long-Term Storage

  • 实验方案

  • 发表日期：

    2026年

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  • Pierre D. M. Reinprecht,
  • Katharina Ebner

摘要

This chapter describes easy and cheap methods for the long-term storage of wild-type and engineered Komagataella phaffii strains. When performed correctly, the outlined cryopreservation protocols allow almost indefinite storage of cells without losing their genetic integrity or viability.