A long-term biocontrol method against parasitic nematodes is provided by endosymbionts, such as symbiotic bacteria like Xenorhabdus and Photorhabdus and entomopathogenic nematodes (EPNs). In this work, endosymbiotic bacteria and fungi are isolated, and their nematicidal activity is screened out. Bacteria are isolated from infected insects, and samples of soils and roots are gathered and exposed to baiting procedures for the purpose of isolating EPNs. On selective media, fungi are cultivated from soils and root samples. Nematicidal efficacy is examined using in vitro and in vivo tests, as well as testing on susceptible plant kinds conducted in polyhouse. The outcomes showed a notable decrease in the need for chemical nematicides and a notable increase in nematode mortality, highlighting the potential of endosymbionts as environmentally friendly biocontrol agents in sustainable agriculture.