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Raman microspectroscopy can be used to analyze live, single endosymbiotic cells with minimal sample preparation, preserving cellular integrity. It is a label-free technique that is ideal for studying endosymbionts, where traditional staining may alter or destroy cellular components. This present chapter describes the detailed preparation of the sample and Raman microscope setup along with short data processing information.

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Single Cell–Based Technologies to Study Endosymbionts: Raman Microspectroscopy

  • 实验方案

  • 发表日期：

    2026年

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  • Komal Chandarana,
  • Natarajan Amaresan

摘要

Raman microspectroscopy can be used to analyze live, single endosymbiotic cells with minimal sample preparation, preserving cellular integrity. It is a label-free technique that is ideal for studying endosymbionts, where traditional staining may alter or destroy cellular components. This present chapter describes the detailed preparation of the sample and Raman microscope setup along with short data processing information.