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The cDNA start codon targeted marker (cDNA-SCoT) methodology is a unique, simple, quick, and efficient method for investigating differential gene expression. The differentially expressed fragments (DEFs) in resistant and susceptible cultivars were identified using cDNA-SCoT profiling. Sanger sequencing and the nBLAST homology search program were used to characterize the functional properties of DEFs. As a result, this methodology is innovative and would be utilized in future studies to identify potential functional genes, which can then be used in marker creation, marker-assisted selection, and uncovering the regulatory role of the relevant genes.

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Oligo-dT Anchored cDNA SCoT: An Innovative Approach for Deciphering Fusarium Wilt Resistance-Related Differential Gene Expression in Chickpea

  • 实验方案

  • 发表日期：

    2026年

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  • S. L. Abhang,
  • P. Sharma,
  • S. N. Datarkar,
  • M. P. Moharil,
  • H. Kudapa,
  • A. P. Ingle,
  • P. V. Jadhav

摘要

The cDNA start codon targeted marker (cDNA-SCoT) methodology is a unique, simple, quick, and efficient method for investigating differential gene expression. The differentially expressed fragments (DEFs) in resistant and susceptible cultivars were identified using cDNA-SCoT profiling. Sanger sequencing and the nBLAST homology search program were used to characterize the functional properties of DEFs. As a result, this methodology is innovative and would be utilized in future studies to identify potential functional genes, which can then be used in marker creation, marker-assisted selection, and uncovering the regulatory role of the relevant genes.