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mRNA vaccines have revolutionized vaccine development against infectious diseases, as demonstrated by the rapid creation and deployment of vaccines for SARS-CoV-2. In this chapter, we present a protocol for the generation of mRNA vaccine formulations utilizing lipid nanoparticle-based delivery systems. Additionally, we provide guidance for the evaluation of (i) mRNA formulation immunogenicity, (ii) mRNA formulation induced antigen cross-presentation, and (iii) antigen-specific CD8+ T-cell responses post in vivo vaccination.

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Generation of Lipid Nanoparticle mRNA Vaccines and Evaluation of Antigen-Specific CD8+ T-Cell Responses

  • 实验方案

  • 发表日期：

    2025年

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  • Ross W. Ward,
  • Aparna Ananthanarayanan,
  • Nina Bhardwaj,
  • Nicolas Vabret

摘要

mRNA vaccines have revolutionized vaccine development against infectious diseases, as demonstrated by the rapid creation and deployment of vaccines for SARS-CoV-2. In this chapter, we present a protocol for the generation of mRNA vaccine formulations utilizing lipid nanoparticle-based delivery systems. Additionally, we provide guidance for the evaluation of (i) mRNA formulation immunogenicity, (ii) mRNA formulation induced antigen cross-presentation, and (iii) antigen-specific CD8+ T-cell responses post in vivo vaccination.