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Humanity has been facing cancer since the beginning of its existence, and the number of new cancer cases grows each year. Immunotherapies based on immune checkpoint inhibition have been intensively developed over the last decade and focus on the blockade of the co-inhibitory molecule complexes such as CTLA-4 with its ligands CD80 and CD86, PD-1 with PD-L1 and PD-L2, and many more. The investigation and development of new immune checkpoints inhibitors is necessary for better cancer treatment. One way of inhibitor evaluation is to assess them in cell-based assays. In this protocol, we focus on describing the cell-based reporter platform for PD-1/PD-L1 inhibitors assessment, which is based on measuring the expression of eGFP under the transcription factor NF-κB, responsible for transcriptional program required for T-cell activation and differentiation.

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Evaluation of Immune Checkpoint Inhibitors in a Jurkat-Based Transcriptional Reporter System: A Cell-Based Reporter Platform for Evaluation of Immune Checkpoint Inhibitors

  • 实验方案

  • 出版模式：

    开放获取

  • 发表日期：

    2026年

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  • Magdalena Bojko,
  • Judith Leitner,
  • Peter Steinberger

摘要

Humanity has been facing cancer since the beginning of its existence, and the number of new cancer cases grows each year. Immunotherapies based on immune checkpoint inhibition have been intensively developed over the last decade and focus on the blockade of the co-inhibitory molecule complexes such as CTLA-4 with its ligands CD80 and CD86, PD-1 with PD-L1 and PD-L2, and many more. The investigation and development of new immune checkpoints inhibitors is necessary for better cancer treatment. One way of inhibitor evaluation is to assess them in cell-based assays. In this protocol, we focus on describing the cell-based reporter platform for PD-1/PD-L1 inhibitors assessment, which is based on measuring the expression of eGFP under the transcription factor NF-κB, responsible for transcriptional program required for T-cell activation and differentiation.