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Macrophage activation has emerged as a key area in immunology, in which they are polarized to adopt different functional phenotypes in response to various environmental signals. Here, we describe the procedure of isolating human primary CD14+ monocytes and polarization toward M1 (pro-inflammatory) and M2 (anti-inflammatory) phenotypes. Relevant cytokine levels can be detected by manual and automated enzyme-linked immunosorbent assays (ELISA).

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Profiling of Macrophage Polarization Using Automated Enzyme-Linked Immunosorbent Assay

  • 实验方案

  • 出版模式：

    开放获取

  • 发表日期：

    2026年

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  • Simon Loevenich,
  • Vu To Nakstad,
  • Hanne Hein Trøen,
  • Margrét Sylvía Sigfúsdóttir,
  • Kristine Sletta,
  • Guro Kruge Nærdal,
  • Håvard Sletta,
  • Geir Klinkenberg,
  • Hanne Haslene-Hox,
  • Torkild Visnes

摘要

Macrophage activation has emerged as a key area in immunology, in which they are polarized to adopt different functional phenotypes in response to various environmental signals. Here, we describe the procedure of isolating human primary CD14+ monocytes and polarization toward M1 (pro-inflammatory) and M2 (anti-inflammatory) phenotypes. Relevant cytokine levels can be detected by manual and automated enzyme-linked immunosorbent assays (ELISA).