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The eukaryotic expression, typically using cultured mammalian cells, is used for stable isotope labeling of glycoproteins. More recently, innovations have been made to reduce the cost of cell culture, control glycoforms, and employ more convenient expression systems other than mammalian cells. Here we describe the procedures involved in expressing isotope-labeled immunoglobulin G by Chinese hamster ovary cells and silkworms.

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Glycoprotein Preparation by Heterologous Expression

  • 实验方案

  • 发表日期：

    2025年

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  • Koichi Kato,
  • Saeko Yanaka,
  • Hirokazu Yagi

摘要

The eukaryotic expression, typically using cultured mammalian cells, is used for stable isotope labeling of glycoproteins. More recently, innovations have been made to reduce the cost of cell culture, control glycoforms, and employ more convenient expression systems other than mammalian cells. Here we describe the procedures involved in expressing isotope-labeled immunoglobulin G by Chinese hamster ovary cells and silkworms.