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We present an innovative method to specifically decrease SUMO2 SUMOylation by using a set of vivo-morpholinos inducing an alternative splicing event that produces a mRNA coding for SUMO2alpha, a non-conjugatable form of SUMO2. This method, also applicable to target SUMO1 and SUMO3, provides an alternative approach to assess the function of the various SUMO paralogs in different cells and the SUMO paralog preference of any given SUMO target.

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A Vivo-Morpholino-Based Approach to Block Paralog-Specific SUMOylation

  • 实验方案

  • 发表日期：

    2025年

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  • Rebeca Orozco-Sepúlveda,
  • Andrea García-Morin,
  • Isabel Gutiérrez-Zubiate,
  • Germán Rosas-Acosta

摘要

We present an innovative method to specifically decrease SUMO2 SUMOylation by using a set of vivo-morpholinos inducing an alternative splicing event that produces a mRNA coding for SUMO2alpha, a non-conjugatable form of SUMO2. This method, also applicable to target SUMO1 and SUMO3, provides an alternative approach to assess the function of the various SUMO paralogs in different cells and the SUMO paralog preference of any given SUMO target.