Contrast-enhanced ultrasound (CEUS) is a powerful imaging modality, increasingly utilized in preclinical research, particularly in murine models. While CEUS offers numerous advantages such as real-time imaging, noninvasiveness, and lack of ionizing radiation, the lack of standardized protocols tailored specifically to the mouse presents a challenge. This work offers a dedicated protocol for CEUS in mice to enhance the reproducibility, reliability, and translatability of preclinical findings. This dedicated protocol addresses the entire CEUS workflow, from contrast agent preparation to animal handling, contrast agent administration, and CEUS imaging parameters. With the proposed standardized workflow establishing guidelines and best practices, researchers can leverage CEUS more effectively, thereby accelerating translatability and the development of innovative approaches to disease characterization, treatment, and management.