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While beneficial to the individual, the asynchronous progression of spermatogenesis makes the evaluation of stage-specific or cell-specific function within the testes challenging for researchers. Here, we describe a method using the aldehyde dehydrogenase inhibitor WIN 18,446 to halt all-trans retinoic acid (atRA) synthesis and prevent the commitment of undifferentiated spermatogonia to meiosis. Following treatment with exogenous atRA after WIN 18,446, individual stages of spermatogenesis can be analyzed with high specificity and this method serves as an invaluable tool to study the progression of spermatogenesis.

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Inhibiting Aldehyde Dehydrogenase Function Using WIN 18,446 to Synchronize Spermatogenesis

  • 实验方案

  • 发表日期：

    2025年

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  • Shelby L. Havel,
  • Michael D. Griswold

摘要

While beneficial to the individual, the asynchronous progression of spermatogenesis makes the evaluation of stage-specific or cell-specific function within the testes challenging for researchers. Here, we describe a method using the aldehyde dehydrogenase inhibitor WIN 18,446 to halt all-trans retinoic acid (atRA) synthesis and prevent the commitment of undifferentiated spermatogonia to meiosis. Following treatment with exogenous atRA after WIN 18,446, individual stages of spermatogenesis can be analyzed with high specificity and this method serves as an invaluable tool to study the progression of spermatogenesis.