Artificial intelligence (AI) proves to be a great tool that makes it possible for machines to learn from experience, regulate to new inputs, and execute human-like activities. AI is already the prime driver of emerging technologies and there are further possibilities in different fields. Certainly, the technology has not yet reached its full effectiveness in many fields but there is one area where AI, integrated with automation, can greatly improve laboratory research known as cell culture. Cell culture is an essential component of present-day biomedical research, allowing researchers to study cellular activities and underlying mechanisms of different diseases in a controlled laboratory setup and environment. Conventional cell culture procedures are hands-on procedures that are time engrossing, susceptible to error and variability, making it difficult to conduct large-scale investigations or screen large numbers of probable drug candidates. Application of AI and automated cell culture procedures can improve the reproducibility of the cell culture protocols that leads to less variability, better conclusions, and finally improving in-depth investigation and drug development. AI algorithms examine data from sensors and cameras to detect alterations in cell behavior, such as changes in appearance, morphology, or growth rate, and make adaptations to the culture conditions in real-time to optimize the growth and health of the cells. Robotic systems automate cell culture tasks, while AI algorithms monitor cell behavior and make adjustments to the culture conditions in real time. AI is considered a significant game changer in 3D cell culture as researchers can grow spheroids or organoids from different cell sources simultaneously, which will ensure consistency and reproducibility. Further, researchers and scientists can create customized workflows and protocols that follow the natural flow of the cell culture process without the need for modularization and human intervention through the use of AI. Though the AI application to cell culture system offers significant change to the scientists, the laboratories, and the companies, there are different aspects of AI applications that need to be considered through vast exploration.