Rice, a staple cereal crop of the family Gramineae, is the second-most cultivated and consumed crop globally. However, asynchronous flowering and limited pollen viability present significant barriers to successful hybridization and genetic exchange between species. Developing efficient pollen storage techniques is critical to overcoming these challenges and improving hybridization success rates. Cryopreservation is a reliable method for conserving pollen (Nuclear Genetic Diversity, NGD) at ultra-low temperatures (−196 °C) in liquid nitrogen, ensuring long-term viability. This technique enables controlled pollination, enhances the success of hybridization, and helps to overcome challenges such as asynchronous flowering and hybridization barriers, ultimately facilitating the production of viable seed sets.