This protocol provides a systematic approach for the collection, drying, and cryopreservation of Alpinia calcarata pollen, along with post-thaw viability assessment. It details optimized procedures for pollen preparation, controlled desiccation, ultra-low temperature storage, and viability evaluation using in vitro germination technique. Effective cryopreservation ensures the long-term preservation of A. calcarata pollen while maintaining its viability and genetic integrity. Key factors influencing success include precise moisture regulation during desiccation and stringent moisture control throughout storage and thawing. Given its significant medicinal value, cryopreservation serves as a crucial tool for conserving high-quality genetic material and facilitating future research and breeding programs.