Cryopreservation of pollen was explored in Abelmoschus moschatus ssp. moschatus (Medik.), a wild species closely related to cultivated okra (A. esculentus (L.) Moench). Asynchronous flowering poses a significant challenge to hybridization between A. esculentus (cultivated okra) and A. moschatus ssp. moschatus (a wild relative). The temporal mismatch in their flowering periods hinders successful cross-pollination and restricts genetic exchange between the two species. To overcome this barrier, it is essential to develop effective methods for pollen storage, enabling the preservation of viable pollen over time. By ensuring pollen availability when needed, such storage techniques can facilitate breeding programs, enabling controlled pollination and hybridization even when flowering is not synchronized. Cryopreservation of pollen at ultra-low temperature facilitates cross-pollination over extended periods, ensuring better synchronization of reproductive phases and improving the success rates of hybridization programs. This chapter provides a comprehensive methodology for the cryopreservation of A. moschatus pollen, with applications extending beyond conservation to its use in hybridization programs.