Hydrocera triflora (L.) Wight & Arn., an aquatic succulent belonging to the family Balsaminaceae, is valued both ecologically and ornamentally. Its vibrant pink flowers have been traditionally used as a natural alternative to henna. Ecologically, this species plays a crucial role in wetland conservation by preventing soil erosion and maintaining habitat stability. Additionally, it has medicinal significance in treating certain skin conditions. Given its ecological and cultural importance, conserving H. triflora is essential for biodiversity protection. Cryopreservation is a widely used technique for the long-term storage of biological materials while maintaining their viability. Preserving the pollen of H. triflora through cryopreservation can support future breeding programs and enhance conservation efforts. This protocol outlines an optimized method for the cryopreservation of H. triflora pollen, ensuring its availability for future research and restoration initiatives.