Cosmos sulphureus and Coreopsis lanceolata are members of the Asteraceae family, and are ecologically and economically significant due to their ornamental value, resilience, and ability to attract pollinators, and prominence in the global cut flower industry. Cryopreserving pollen at ultra-low temperatures, typically in liquid nitrogen, facilitates the long-term storage of genetic material, making it available for future breeding, conservation, and restoration efforts. C. sulphureus is particularly recognized for its medicinal properties and bioactive compounds, while C. lanceolata is valued for its drought resistance and pollinator attraction. Despite their ecological and agricultural benefits, both species face challenges such as invasiveness and potential negative impacts on crop systems. Pollen cryopreservation offers a vital tool for preserving the genetic resources of these species, ensuring their continued utility in climate change mitigation and future breeding programs.