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The respiratory syncytial virus (RSV) minigenome system is a powerful analytical tool that has enabled viral transcription and genome replication to be modeled in a cellular context without viral infection. The minigenome assay can be used to dissect the different steps in RSV transcription and genome replication, assess the effects of mutations in cis-acting RNA elements, analyze mutations in the polymerase and other proteins in the transcription–replication machinery, and assess the effect of antiviral compounds on RSV polymerase functions. Here, we describe the RSV minigenome assay and detail a northern blotting technique for detecting viral mRNA transcripts and genome replication products.

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Minigenome System to Study Respiratory Syncytial Virus Transcription Versus Genome Replication

  • 实验方案

  • 发表日期：

    2025年

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  • Heesu Kim,
  • Sarah L. Noton

摘要

The respiratory syncytial virus (RSV) minigenome system is a powerful analytical tool that has enabled viral transcription and genome replication to be modeled in a cellular context without viral infection. The minigenome assay can be used to dissect the different steps in RSV transcription and genome replication, assess the effects of mutations in cis-acting RNA elements, analyze mutations in the polymerase and other proteins in the transcription–replication machinery, and assess the effect of antiviral compounds on RSV polymerase functions. Here, we describe the RSV minigenome assay and detail a northern blotting technique for detecting viral mRNA transcripts and genome replication products.