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Serial adaptation of viruses to inhibitors enhances the understanding of the mechanism of inhibition, helps to identify physical target sites of compounds, and serves as a test for the genetic barrier to reducing virus susceptibility. Virus adaptations can be carried out in vivo or in tissue culture using sub-inhibitory concentrations of compounds that are increased with each passage of the virus or kept static. Here we describe the method of serially adapting a virus in tissue culture.

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Serial Adaptation of Viruses for Reduced Susceptibility Profiling

  • 实验方案

  • 发表日期：

    2025年

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  • Michael R. Sirrine,
  • Richard K. Plemper

摘要

Serial adaptation of viruses to inhibitors enhances the understanding of the mechanism of inhibition, helps to identify physical target sites of compounds, and serves as a test for the genetic barrier to reducing virus susceptibility. Virus adaptations can be carried out in vivo or in tissue culture using sub-inhibitory concentrations of compounds that are increased with each passage of the virus or kept static. Here we describe the method of serially adapting a virus in tissue culture.