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We describe the method of collection and microinjection of oocytes, acquisition of images of the oocyte cortex, as well as our image analysis pipeline to quantify the F-actin bundles in the oocyte cortex from these images. The metric is based on spatial correlations of the image intensity gradient.

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Imaging and Quantifying F-Actin Bundles in the Cortex of Mammalian Oocytes

  • 实验方案

  • 发表日期：

    2025年

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  • Ghislain de Labbey,
  • Elvira Nikalayevich

摘要

We describe the method of collection and microinjection of oocytes, acquisition of images of the oocyte cortex, as well as our image analysis pipeline to quantify the F-actin bundles in the oocyte cortex from these images. The metric is based on spatial correlations of the image intensity gradient.