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Confocal microscopy provides information on host–pathogen interactions. To examine infection at the respiratory epithelial barrier, primary bronchial cells differentiated at an air-liquid interface (ALI) can be imaged and reconstructed as a 3D model. In this chapter, we describe a method for immunostaining and fluorescence imaging of well-differentiated bronchial epithelial tissue that has been exposed to the cystic fibrosis respiratory pathogen Achromobacter xylosoxidans.

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Investigating Bacterial Invasion of Highly Differentiated Human Bronchial Epithelial Cell Barriers

  • 实验方案

  • 发表日期：

    2025年

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  • Hannah J. Parks,
  • Miguel A. Valvano

摘要

Confocal microscopy provides information on host–pathogen interactions. To examine infection at the respiratory epithelial barrier, primary bronchial cells differentiated at an air-liquid interface (ALI) can be imaged and reconstructed as a 3D model. In this chapter, we describe a method for immunostaining and fluorescence imaging of well-differentiated bronchial epithelial tissue that has been exposed to the cystic fibrosis respiratory pathogen Achromobacter xylosoxidans.