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The handling of high-molecular-weight mucins requires specialized methods. The present manuscript introduces a slot blotting-based technique to screen post-ultracentrifugation fractions using colorimetric assays. Next, we describe an easy slot blot-based method to characterize mucin-bacteria interactions and to assess the effects of glycan enzymatic treatment on bacterial adhesion.

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Mucins and Bacterial Interactions

  • 实验方案

  • 发表日期：

    2025年

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  • Yannick Rossez,
  • Renaud Léonard,
  • Olivier Vidal,
  • Zina Chouit,
  • Yann Guerardel,
  • Edwige Madec,
  • Catherine Robbe-Masselot

摘要

The handling of high-molecular-weight mucins requires specialized methods. The present manuscript introduces a slot blotting-based technique to screen post-ultracentrifugation fractions using colorimetric assays. Next, we describe an easy slot blot-based method to characterize mucin-bacteria interactions and to assess the effects of glycan enzymatic treatment on bacterial adhesion.