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High-throughput yeast two-hybrid (YTH) screening systems are essential technologies for understanding the viral life cycle, pathogenesis, and development of antiviral therapies. They are commonly used in molecular virology to identify and analyze the complex molecular interactions between viruses and host cells. This chapter details the relevant information and experimental procedures for systematically screening and identifying interactions between viral and host proteins via high-throughput YTH technology.

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High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology

  • 实验方案

  • 发表日期：

    2025年

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  • Sisi Xia,
  • Jialin Wang,
  • Xiaoping Huo,
  • Chunfu Zheng,
  • Hongjian Li,
  • Jun Chen

摘要

High-throughput yeast two-hybrid (YTH) screening systems are essential technologies for understanding the viral life cycle, pathogenesis, and development of antiviral therapies. They are commonly used in molecular virology to identify and analyze the complex molecular interactions between viruses and host cells. This chapter details the relevant information and experimental procedures for systematically screening and identifying interactions between viral and host proteins via high-throughput YTH technology.