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Reactive oxygen species (ROS)Reactive oxygen species (ROS) play an important role in the differentiation of immature stem cells to mature adipocytes in a process known as adipogenesisAdipocytes. Despite decades of study, there is still a gap in our understanding of the exact mechanisms played by ROSReactive oxygen species (ROS) in regulating adipogenesisAdipogenesisAdipogenesis. This chapter describes flow cytometric measurement of endogenous ROSReactive oxygen species (ROS) production during adipogenesisAdipogenesis in adipose-derived stromal/stem cells (ASCsAdipose-derived stem/stromal cells (ASCs)).

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Measurement of Intracellular Reactive Oxygen Species During Adipogenesis

  • 实验方案

  • 发表日期：

    2025年

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  • Ladislaus Tapiwa Adam,
  • Melvin A. Ambele,
  • Michael S. Pepper

摘要

Reactive oxygen species (ROS)Reactive oxygen species (ROS) play an important role in the differentiation of immature stem cells to mature adipocytes in a process known as adipogenesisAdipocytes. Despite decades of study, there is still a gap in our understanding of the exact mechanisms played by ROSReactive oxygen species (ROS) in regulating adipogenesisAdipogenesisAdipogenesis. This chapter describes flow cytometric measurement of endogenous ROSReactive oxygen species (ROS) production during adipogenesisAdipogenesis in adipose-derived stromal/stem cells (ASCsAdipose-derived stem/stromal cells (ASCs)).