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Adipogenesis is a complex multiphase process in which preadipocytes develop into adipocytes. This tightly regulated process is facilitated by a cascade of genes and transcription factors. In this chapter, we describe a protocol that can be used to quantify the expression of adipogenic markers using RT-qPCR.

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RT-qPCR Quantification of Adipogenic Marker Genes (PPARg, C/EBPa, FABP4, and CD36)

  • 实验方案

  • 发表日期：

    2025年

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  • Carina C. Babbo,
  • Reabetswe R. Pitere,
  • Rachel Giles,
  • Melvin A. Ambele,
  • Michael S. Pepper

摘要

Adipogenesis is a complex multiphase process in which preadipocytes develop into adipocytes. This tightly regulated process is facilitated by a cascade of genes and transcription factors. In this chapter, we describe a protocol that can be used to quantify the expression of adipogenic markers using RT-qPCR.