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Adipose-derived stromal/stem cells (ASCs) have raised a great deal of interest in research and regenerative medicine with potential applications in the treatment of several diseases. ASCs are often required in large numbers for use in research and clinical applications. Standardization of isolation methods, expansion, and characterization will ensure quality of the cells as well as safety in their use. This protocol describes the expansion and characterization of ASCs and highlights ASC viability assessment following expansion.

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In Vitro Expansion of Human Adipose-Derived Stem Cells (ASCs)

  • 实验方案

  • 发表日期：

    2025年

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  • Ladislaus Tapiwa Adam,
  • Melvin A. Ambele,
  • Michael S. Pepper

摘要

Adipose-derived stromal/stem cells (ASCs) have raised a great deal of interest in research and regenerative medicine with potential applications in the treatment of several diseases. ASCs are often required in large numbers for use in research and clinical applications. Standardization of isolation methods, expansion, and characterization will ensure quality of the cells as well as safety in their use. This protocol describes the expansion and characterization of ASCs and highlights ASC viability assessment following expansion.